ARG65394

anti-CD116 antibody [4H1]

anti-CD116 antibody [4H1] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot and Human

Developmental Biology antibody; Immune System antibody
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Overview

Product Description Mouse Monoclonal antibody [4H1] recognizes CD116
Tested Reactivity Hu
Tested Application CyTOF®-candidate, FACS, IHC-Fr, IP, WB
Specificity The clone 4H1 recognizes human CD116, the GM-CSF receptor alpha subunit (approx. 80 kDa) expressed e.g. by neutrophils, eosinophils, monocytes and macrophages.
Host Mouse
Clonality Monoclonal
Clone 4H1
Isotype IgG1
Target Name CD116
Immunogen CD116-transfected COS cells
Conjugation Un-conjugated
Alternate Names Granulocyte-macrophage colony-stimulating factor receptor subunit alpha; GMCSFR; CDw116; GM-CSF-R-alpha; CD antigen CD116; CSF2R; CD116; GMR-alpha; CSF2RAX; CSF2RAY; GMCSFR-alpha; GMR; SMDP4; CSF2RX; CSF2RY

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 4 µg/ml
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer TBS (pH 8.0) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1438 Human CSF2RA

Swiss-port # P15509 Human Granulocyte-macrophage colony-stimulating factor receptor subunit alpha

Gene Symbol CSF2RA
Gene Full Name colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
Background CD116 (GM-CSF R alpha) is the low affinity receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF). CD116 heterodimerizes with CD131, the common beta chain subunit shared with IL-3 and IL5 receptors, to form the high affinity GM-CSF receptor. CD116 is expressed by myeloid cells including macrophages, neutrophils, eosinophils, dendritic cells, and their precursors, as well as on endothelial cells. It is being used as a specific marker of myeloid leukemias.
Function Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. [UniProt]
Highlight Related products:
CD116 antibodies; CD116 ELISA Kits; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 46 kDa

Images (2) Click the Picture to Zoom In

  • ARG65394 anti-CD116 antibody [4H1] FACS image

    Flow Cytometry: Human peripheral blood stained with ARG65394 anti-CD116 antibody [4H1] at 3 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.

  • ARG65394 anti-CD116 antibody [4H1] FACS image

    Flow Cytometry: Separation of human monocytes (red-filled) from lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65394 anti-CD116 antibody [4H1] at 3 µg/ml dilution, followed by PE-conjugated Goat anti-Mouse antibody.

Customer's Feedback

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Review for anti-CD116 antibody [4H1]

Application:WB

Sample:293T

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:300

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC