ARG42424

anti-CD109 antibody [W7C5] (PE)

anti-CD109 antibody [W7C5] (PE) for Flow cytometry and Human

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [W7C5] recognizes CD109
Tested Reactivity Hu
Tested Application FACS
Specificity The mouse monoclonal antibody W7C5 recognizes CD109, an approximately 165 kDa GPI-anchored extracellular protein expressed mainly on various hematopoietic cells, activated T lymphoblasts, activated platelets, and endothelial cells.
Host Mouse
Clonality Monoclonal
Clone W7C5
Isotype IgG1
Target Name CD109
Antigen Species Human
Immunogen WERI-Rb-1 retinoblastoma cell line.
Conjugation PE
Alternate Names p180; CPAMD7; Platelet-specific Gov antigen; CD109 antigen; r150; 150 kDa TGF-beta-1-binding protein; CD antigen CD109; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 135228 Human CD109

Swiss-port # Q6YHK3 Human CD109 antigen

Gene Symbol CD109
Gene Full Name CD109 molecule
Background This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
Function Modulates negatively TGFB1 signaling in keratinocytes. [UniProt]
Cellular Localization Cell membrane; Lipid-anchor, GPI-anchor. [UniProt]
Calculated MW 162 kDa
PTM N-glycosylated.

2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG42424 anti-CD109 antibody [W7C5] (PE) FACS image

    Flow Cytometry: Human PHA stimulated peripheral blood mononuclear cells stained with ARG42424 anti-CD109 antibody [W7C5] (PE) at 10 µl / 10^6 cells in 100 µl of cell suspension.

  • ARG42424 anti-CD109 antibody [W7C5] (PE) FACS image

    Flow Cytometry: Separation of Human CD109 positive cells (red-filled) from CD109 negative cells (black-dashed). Human PHA stimulated peripheral blood mononuclear cells stained with ARG42424 anti-CD109 antibody [W7C5] (PE) at 10 µl / 10^6 cells in 100 µl of cell suspension.