ARG42423
anti-CD109 antibody [W7C5]
anti-CD109 antibody [W7C5] for Flow cytometry and Human
Overview
| Product Description | Mouse Monoclonal antibody [W7C5] recognizes CD109 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The mouse monoclonal antibody W7C5 recognizes CD109, an approximately 165 kDa GPI-anchored extracellular protein expressed mainly on various hematopoietic cells, activated T lymphoblasts, activated platelets, and endothelial cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | W7C5 |
| Isotype | IgG1 |
| Target Name | CD109 |
| Antigen Species | Human |
| Immunogen | WERI-Rb-1 retinoblastoma cell line. |
| Conjugation | Un-conjugated |
| Alternate Names | p180; CPAMD7; Platelet-specific Gov antigen; CD109 antigen; r150; 150 kDa TGF-beta-1-binding protein; CD antigen CD109; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD109 |
| Gene Full Name | CD109 molecule |
| Background | This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014] |
| Function | Modulates negatively TGFB1 signaling in keratinocytes. [UniProt] |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. [UniProt] |
| Calculated MW | 162 kDa |
| PTM | N-glycosylated. 2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG42423 anti-CD109 antibody [W7C5] FACS image
Flow Cytometry: Human peripheral blood stained with ARG42423 anti-CD109 antibody [W7C5] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG42423 anti-CD109 antibody [W7C5] FACS image
Flow Cytometry: Separation of Human monocytes (red-filled) from CD109 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG42423 anti-CD109 antibody [W7C5] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
