ARG22265
anti-CD107b / LAMP2 antibody [GL2A7]
anti-CD107b / LAMP2 antibody [GL2A7] for Flow cytometry,ICC/IF,Immunohistochemistry (PFA perfusion fixed frozen sections),IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rabbit
Overview
| Product Description | Rat Monoclonal antibody [GL2A7] recognizes CD107b / LAMP2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rb |
| Tested Application | FACS, ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, IP, WB |
| Specificity | Detects ~100-110kDa. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | GL2A7 |
| Isotype | IgG1 |
| Target Name | CD107b / LAMP2 |
| Antigen Species | Mouse |
| Immunogen | Purified preparation of Mouse liver lysosomal membranes |
| Conjugation | Un-conjugated |
| Alternate Names | CD antigen CD107b; LAMPB; CD107b; LGP110; Lysosome-associated membrane glycoprotein 2; LAMP-2; CD107 antigen-like family member B; Lysosome-associated membrane protein 2 |
Application Instructions
| Application Suggestion |
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| Application Note | For best ICC/IF results, fix cells with methanol or with (para)formaldehyde-lysine-periodate formulation; for the latter, permeabilize with saponin, not with Triton X-100 or NP-40. Unfixed cells that have been permeabilized with saponin can also be stained. For immunoprecipitation, the antigen solubilized with Triton X-100 will bind to GL2A7 coupled to a matrix. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 100-110 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P13473 Human Lysosome-associated membrane glycoprotein 2 Swiss-port # P17047 Mouse Lysosome-associated membrane glycoprotein 2 |
|---|---|
| Gene Symbol | Lamp2 |
| Gene Full Name | lysosomal-associated membrane protein 2 |
| Background | The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008] |
| Function | Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. [UniProt] |
| Cellular Localization | Cell membrane, Endosome, Endosome membrane, Lysosome, Lysosome membrane |
| Calculated MW | 45 kDa |
| PTM | O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans. |
Images (1) Click the Picture to Zoom In
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ARG22265 anti-CD107b / LAMP2 antibody [GL2A7] WB image
Western blot: 1) MW ladder, 2) 20 µg of HEK293, and 3) 10 µg of NIH3T3 lysates stained with ARG22265 anti-CD107b / LAMP2 antibody [GL2A7] at 1:500 dilution (60 min at RT). Block: 5% milk + TBST 1hr at RT.
