ARG65453
anti-CD107a / LAMP1 antibody [H4A3] (azide free)
anti-CD107a / LAMP1 antibody [H4A3] (azide free) for Electron microscopy,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Purification,Western blot and Human,Mouse,Rat,Primates
Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody
Overview
| Product Description | Azide free Mouse Monoclonal antibody [H4A3] recognizes CD107a / LAMP1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, NHuPrm |
| Tested Application | EM, FACS, ICC/IF, IHC-Fr, IHC-P, IP, Puri, WB |
| Specificity | The mouse monoclonal antibody H4A3 recognizes LAMP1 / CD107a, an approximately 100120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | H4A3 |
| Isotype | IgG1 |
| Target Name | CD107a / LAMP1 |
| Antigen Species | Human |
| Immunogen | Human PBMC |
| Conjugation | Un-conjugated |
| Alternate Names | LGP120; CD107a; LAMPA; CD antigen CD107a; Lysosome-associated membrane glycoprotein 1; CD107 antigen-like family member A; LAMP-1; Lysosome-associated membrane protein 1 |
Application Instructions
| Application Suggestion |
|
||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | BBS (pH 8.2) |
| Concentration | 0.1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LAMP1 |
| Gene Full Name | lysosomal-associated membrane protein 1 |
| Background | CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation. |
| Function | Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. [UniProt] |
| Highlight | Related products: LAMP1 antibodies; LAMP1 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Baking soda restores circadian clock in tumor cells |
| Research Area | Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody |
| Calculated MW | 45 kDa |
| PTM | O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells. |
Images (2) Click the Picture to Zoom In
-
ARG65453 anti-CD107a / LAMP1 antibody [H4A3] (azide free) ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG65453 anti-CD107a / LAMP1 antibody [H4A3] (azide free) at 1:50 dilution.
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
-
ARG65453 anti-CD107a / LAMP1 antibody [H4A3] (azide free) WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG65453 anti-CD107a / LAMP1 antibody [H4A3] (azide free) at 1:1000 dilution.
Customer's Feedback
Excellent
Review for anti-CD107a / LAMP1 antibody [H4A3] (azide free)
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：20 - 25 ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:50
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Excellent
Review for anti-CD107a / LAMP1 antibody [H4A3] (azide free)
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Clone References
Cellular mechanisms of toll-like receptor-3 activation in the thalamus are associated with white matter injury in the developing brain.
The role of the helper lipid on the DNA transfection efficiency of lipopolyplex formulations.
Induced pluripotent stem cells restore function in a human cell loss model of open-angle glaucoma.
Glutamine deprivation stimulates mTOR-JNK-dependent chemokine secretion.
ICC/IF / Human
QSOX1 inhibits autophagic flux in breast cancer cells.
ICC/IF / Human
Filamin A promotes dynamin-dependent internalization of hyperpolarization-activated cyclic nucleotide-gated type 1 (HCN1) channels and restricts Ih in hippocampal neurons.
ICC/IF / Rat
Blood-brain barrier impairment in MPS III patients.
IHC-Fr / Human
Pathogenic Parkinson's disease mutations across the functional domains of LRRK2 alter the autophagic/lysosomal response to starvation.
WB / Human
Autophagy in retinal ganglion cells in a rhesus monkey chronic hypertensive glaucoma model.
WB / Rhesus monkey
Hypertonic stress increases claudin-4 expression and tight junction integrity in association with MUPP1 in IMCD3 cells.
ICC/IF / Mouse
