ARG22180

anti-CD107a / LAMP1 antibody [H4A3] (FITC)

anti-CD107a / LAMP1 antibody [H4A3] (FITC) for Electron microscopy,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,African green monkey,Macaque,Rhesus Monkey

Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [H4A3] recognizes CD107a / LAMP1
Tested Reactivity Hu, AGMK, Macaq, R. Mk
Tested Application EM, FACS, ICC/IF, IHC-Fr, IHC-P, WB
Specificity Human/Rhesus/African Green Monkey/Pigtail Macaque/Sooty Mangabey CD107a
Host Mouse
Clonality Monoclonal
Clone H4A3
Isotype IgG1, kappa
Target Name CD107a / LAMP1
Antigen Species Human
Immunogen Human adherent peripheral blood cells
Conjugation FITC
Alternate Names LGP120; CD107a; LAMPA; CD antigen CD107a; Lysosome-associated membrane glycoprotein 1; CD107 antigen-like family member A; LAMP-1; Lysosome-associated membrane protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
EMAssay-dependent
FACS10 µl/10^6 cells
ICC/IF1:50
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3916 Human LAMP1

Swiss-port # P11279 Human Lysosome-associated membrane glycoprotein 1

Gene Symbol LAMP1
Gene Full Name lysosomal-associated membrane protein 1
Background The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq, Jul 2008]
Function Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. [UniProt]
Research Area Cancer antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody; Lysosome Marker antibody
Calculated MW 45 kDa
PTM O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells.

Images (2) Click the Picture to Zoom In

  • ARG22180 anti-CD107a / LAMP1 antibody [H4A3] (FITC) ICC/IF image

    Immunofluorescence: Human myeloid dendritic cells fixed with 4% paraformaldehyde for 15 min, permeabilized with PBS containing 100 µg/ml of digitonin and 1% BSA for 30 min. Cells were stained with ARG22180 anti-CD107a / LAMP1 antibody [H4A3] (FITC) at 1:50 dilution.

  • ARG22180 anti-CD107a / LAMP1 antibody [H4A3] (FITC) FACS image

    Flow Cytometry: Human T cell leukemia cell line Jurkat was intracellularly stained with ARG22180 anti-CD107a / LAMP1 antibody [H4A3] (FITC).