ARG42059

anti-CD106 / VCAM1 antibody

anti-CD106 / VCAM1 antibody for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes CD106 / VCAM1
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD106 / VCAM1
Antigen Species Human
Immunogen Synthetic peptide of Human CD106 / VCAM1.
Conjugation Un-conjugated
Alternate Names CD106; INCAM-100; Vascular cell adhesion protein 1; VCAM-1; CD antigen CD106; V-CAM 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:50 - 1:200
IHC-Fr1:50 - 1:200
IHC-P1:50 - 1:200
IP1:20
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse kidney
Observed Size ~ 100 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22329 Mouse VCAM1

GeneID: 25361 Rat VCAM1

GeneID: 7412 Human VCAM1

Gene Symbol VCAM1
Gene Full Name vascular cell adhesion molecule 1
Background This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]
Function Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 81 kDa
PTM Sialoglycoprotein. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG42059 anti-CD106 / VCAM1 antibody IHC-Fr image

    Immunohistochemistry: Mouse endothelial stained with ARG42059 anti-CD106 / VCAM1 antibody at 1:100 dilution.

    From Rui Fan et al. Biochim Biophys Acta Mol Cell Biol Lipids. (2023), doi: 10.1016/j.bbalip.2023.159330, Fig. 2B.

  • ARG42059 anti-CD106 / VCAM1 antibody ICC/IF image

    Immunofluorescence: K562 cells stained with ARG42059 anti-CD106 / VCAM1 antibody.

  • ARG42059 anti-CD106 / VCAM1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human tonsil tissue stained with ARG42059 anti-CD106 / VCAM1 antibody.

  • ARG42059 anti-CD106 / VCAM1 antibody WB image

    Western blot: Mouse kidney lysate stained with ARG42059 anti-CD106 / VCAM1 antibody.

Specific References

Severe hypertriglyceridemia caused by Gpihbp1 deficiency facilitates vascular remodeling through increasing endothelial activation and oxidative stress

IHC-Fr / Mouse

Rui Fan et al.
Biochim Biophys Acta Mol Cell Biol Lipids.,  (2023)

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