ARG65507

anti-CD105 / Endoglin antibody [MJ7/18]

anti-CD105 / Endoglin antibody [MJ7/18] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot and Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody

Overview

Product Description Rat Monoclonal antibody [MJ7/18] recognizes CD105 / Endoglin
Tested Reactivity Ms
Tested Application FACS, IHC-Fr, IP, WB
Host Rat
Clonality Monoclonal
Clone MJ7/18
Isotype IgG2a, kappa
Target Name CD105 / Endoglin
Antigen Species Mouse
Immunogen Inflamed mouse skin
Conjugation Un-conjugated
Alternate Names CD antigen CD105; HHT1; Endoglin; ORW1; END

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 1 µg/10^6 cells
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13805 Mouse ENG

Swiss-port # Q63961 Mouse Endoglin

Gene Symbol Eng
Gene Full Name endoglin
Background CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
Function Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody
Calculated MW 71 kDa

Images (1) Click the Picture to Zoom In

  • ARG65507 anti-CD105 / Endoglin antibody [MJ7/18] FACS image

    Flow Cytometry: BALB/c Mouse endothelioma cell line bEnd.3 was stained with ARG65507 anti-CD105 / Endoglin antibody [MJ7/18] followed by ARG21704 Mouse anti-Rat IgG2a antibody [2A8F4] (PE).

Clone References

Osteogenic Potential of Mouse Adipose-Derived Stem Cells Sorted for CD90 and CD105 In Vitro.

FACS / Mouse

Yamamoto M et al.
Stem Cells Int.,  (2014)

publication_link

 

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A role for endoglin as a suppressor of malignancy during mouse skin carcinogenesis.

WB / 

Pérez-Gómez E et al.
Cancer Res.,  (2007)

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Monitoring response to anticancer therapy by targeting microbubbles to tumor vasculature.

IHC-Fr / 

Korpanty G et al.
Clin Cancer Res.,  (2007)

publication_link

 

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Liver sinusoidal endothelial cells tolerize T cells across MHC barriers in mice.

IHC-Fr / 

Onoe T et al.
J Immunol.,  (2005)

publication_link

 

hr_line

Expression of the TGF-beta coreceptor endoglin in epidermal keratinocytes and its dual role in multistage mouse skin carcinogenesis.

WB, IHC-Fr / 

Quintanilla M et al.
Oncogene.,  (2003)

publication_link

 

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