ARG65417

anti-CD103 / Integrin alpha E antibody [Ber-ACT8]

anti-CD103 / Integrin alpha E antibody [Ber-ACT8] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Primates

Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [Ber-ACT8] recognizes CD103 / Integrin alpha E
Tested Reactivity Hu, NHuPrm
Tested Application CyTOF®-candidate, FACS, IHC-Fr, IP, WB
Specificity The clone Ber-ACT8 recognizes CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes.
HLDA V; WS Code A067
Host Mouse
Clonality Monoclonal
Clone Ber-ACT8
Isotype IgG1
Target Name CD103 / Integrin alpha E
Antigen Species Human
Immunogen HTLV-1 induced human T cell line MAPS16
Conjugation Un-conjugated
Alternate Names CD103; HUMINAE; Integrin alpha-E; Mucosal lymphocyte 1 antigen; CD antigen CD103; HML-1 antigen; Integrin alpha-IEL

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1 - 4 µg/ml
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3682 Human ITGAE

Swiss-port # P38570 Human Integrin alpha-E

Gene Symbol ITGAE
Gene Full Name integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)
Background CD103 / Integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation.
Function Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. [UniProt]
Highlight Related products:
CD103 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 130 kDa