ARG55707

anti-CD10 antibody

anti-CD10 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CD10
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD10
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 492-525 (Center) of Human CD10.
Conjugation Un-conjugated
Alternate Names Enkephalinase; Neutral endopeptidase; Neutral endopeptidase 24.11; Common acute lymphocytic leukemia antigen; Neprilysin; CD antigen CD10; CALLA; EC 3.4.24.11; CD10; Skin fibroblast elastase; SFE; Atriopeptidase; NEP

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:25
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control LNCaP

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) sodium azide.
Preservative 0.09% (W/V) sodium azide.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17380 Mouse MME

GeneID: 24590 Rat MME

GeneID: 4311 Human MME

Gene Symbol MME
Gene Full Name membrane metallo-endopeptidase
Background CD10 is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. [provided by RefSeq, Aug 2017]
Function CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573). [UniProt]
Cellular Localization Cell membrane; Single-pass type II membrane protein
Calculated MW 86 kDa
PTM Myristoylation is a determinant of membrane targeting.
Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity.

Images (2) Click the Picture to Zoom In

  • ARG55707 anti-CD10 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue stained with ARG55707 anti-CD10 antibody at 1:25 dilution.

  • ARG55707 anti-CD10 antibody WB image

    Western blot: 35 µg of LNCaP cell lysate stained with ARG55707 anti-CD10 antibody at 1:1000 dilution.