ARG22455
anti-CCT7 / TCP1 eta antibody [PK/16/8/a]
anti-CCT7 / TCP1 eta antibody [PK/16/8/a] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Bovine,Rabbit
Overview
| Product Description | Rat Monoclonal antibody [PK/16/8/a] recognizes CCT7 / TCP1 eta This antibody recognizes the eta polypeptide of the CCT chaperonin molecule complex.CCTη is a 543 amino acid ~60kDa molecule encoded by the CCT7 gene, a member of the TCP-1 chaperonin family (UniProt: Q2NKZ1)The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.Rat anti CCTeta antibody, clone PK/16/8/a detects a band of ~60kDa in HeLa cell lysates using western blotting under reducing conditions. |
|---|---|
| Tested Reactivity | Hu, Ms, Bov, Rb |
| Tested Application | IHC-P, WB |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | PK/16/8/a |
| Isotype | IgG2a |
| Target Name | CCT7 / TCP1 eta |
| Antigen Species | Bovine |
| Conjugation | Un-conjugated |
| Alternate Names | TCP1ETA; CCT-eta; CCTETA; T-complex protein 1 subunit eta; CCTH; TCP-1-eta; NIP7-1; HIV-1 Nef-interacting protein |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Western Blotting: MCA2179 detects a band of approximately 60kD in Hela cell lysates. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCT7 |
| Gene Full Name | chaperonin containing TCP1, subunit 7 (eta) |
| Background | This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 6. [provided by RefSeq, Oct 2009] |
| Function | Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin (By similarity). [UniProt] |
| Calculated MW | 59 kDa |
Images (1) Click the Picture to Zoom In
-
ARG22455 anti-CCT7 / TCP1 eta antibody [PK/16/8/a] WB image
Western blot: 1) HeLa whole cell lysate and 2) HeLa heat stressed whole cell lysate stained with ARG22455 anti-CCT7 / TCP1 eta antibody [PK/16/8/a].
