ARG64951
anti-CCT3 / TCP1 antibody
anti-CCT3 / TCP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CCT3 / TCP1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Rat, Dog, Pig |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize all reported isoforms (NP_005989.3; NP_001008883.1; NP_001008800.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CCT3 / TCP1 |
| Antigen Species | Human |
| Immunogen | C-GHKKKGDDQSRQGG |
| Conjugation | Un-conjugated |
| Alternate Names | CCT-gamma; PIG48; TRIC5; TCP-1-gamma; T-complex protein 1 subunit gamma; CCTG; hTRiC5 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8. [provided by RefSeq, Aug 2010] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 61 kDa |
Images (2) Click the Picture to Zoom In
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ARG64951 anti-CCT3 / TCP1 antibody WB image
Western Blot: Mouse Testis lysate (35 µg protein in RIPA buffer) stained with ARG64951 anti-CCT3 / TCP1 antibody at 0.01 µg/ml dilution.
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ARG64951 anti-CCT3 / TCP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64951 anti-CCT3 / TCP1 antibody at 5 µg/ml dilution followed by AP-staining.
