ARG66004
anti-CCL4 / MIP1 beta antibody
anti-CCL4 / MIP1 beta antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CCL4 / MIP1 beta |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC-P, Neut, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CCL4 / MIP1 beta |
| Antigen Species | Human |
| Immunogen | E. coli derived recombinant Human CCL4 / MCP1 beta. (APMGSDPPTA CCFSYTARKL PHNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN) |
| Conjugation | Un-conjugated |
| Alternate Names | SCYA4L; Lymphocyte activation gene 1 protein; MIP-1-beta; Macrophage inflammatory protein 1-beta; LAG-1; LAG1; CCL4L; Monocyte adherence-induced protein 5-alpha; Small-inducible cytokine A4-like; SCYA4L1; C-C motif chemokine 4-like; AT744.2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CCL4L1 |
|---|---|
| Gene Full Name | chemokine (C-C motif) ligand 4-like 1 |
| Background | This gene is one of several cytokine genes that are clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins that function in inflammatory and immunoregulatory processes. The protein encoded by this family member is similar to the chemokine (C-C motif) ligand 4 product, which inhibits HIV entry by binding to the cellular receptor CCR5. The copy number of this gene varies among individuals, where most individuals have one to five copies. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| Function | Chemokine that induces chemotaxis of cells expressing CCR5 or CCR1. Inhibits HIV replication in peripheral blood monocytes that express CCR5. [UniProt] |
| Calculated MW | 10 kDa |
| PTM | N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes. |
Images (4) Click the Picture to Zoom In
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ARG66004 anti-CCL4 / MIP1 beta antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human pancreas adenocarcinoma. The recommended ARG66004 anti-CCL4 / MIP1 beta antibody concentration is 1.0 µg/ml with 2 hour incubation at RT. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG66004 anti-CCL4 / MIP1 beta antibody WB image
Western blot: 250 - 0.24 ng of Human MIP-1β stained with ARG66004 anti-CCL4 / MIP1 beta antibody, under non-reducing conditions.
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ARG66004 anti-CCL4 / MIP1 beta antibody standard curve image
Sandwich ELISA: ARG66004 anti-CCL4 / MIP1 beta antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66005 anti-CCL4 / MCP1 beta antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG66004 anti-CCL4 / MIP1 beta antibody WB image
Western blot: 250 - 0.24 ng of Human MIP-1β stained with ARG66004 anti-CCL4 / MIP1 beta antibody, under reducing conditions.
