ARG66142

anti-CCL28 antibody

anti-CCL28 antibody for ELISA,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes CCL28
Tested Reactivity Hu
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CCL28
Antigen Species Human
Immunogen E. coli derived recombinant Human CCL28.
(SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY)
Conjugation Un-conjugated
Alternate Names SCYA28; MEC; Mucosae-associated epithelial chemokine; CCK1; C-C motif chemokine 28; Small-inducible cytokine A28; Protein CCK1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66143 as a detection antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56477 Human CCL28

Swiss-port # Q9NRJ3 Human C-C motif chemokine 28

Gene Symbol CCL28
Gene Full Name chemokine (C-C motif) ligand 28
Background This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for resting CD4 or CD8 T cells and eosinophils. The product of this gene binds to chemokine receptors CCR3 and CCR10. This chemokine may play a role in the physiology of extracutaneous epithelial tissues, including diverse mucosal organs. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
Function Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. [UniProt]
Calculated MW 14 kDa

Images (3) Click the Picture to Zoom In

  • ARG66142 anti-CCL28 antibody WB image

    Western blot: 250 - 0.24 ng of Human MEC stained with ARG66142 anti-CCL28 antibody, under reducing conditions.

  • ARG66142 anti-CCL28 antibody standard curve image

    Sandwich ELISA: ARG66142 anti-CCL28 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66143 anti-CCL28 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66142 anti-CCL28 antibody WB image

    Western blot: 250 - 0.24 ng of Human MEC stained with ARG66142 anti-CCL28 antibody, under non-reducing conditions.