ARG66136

anti-CCL27 / CTACK antibody

anti-CCL27 / CTACK antibody for ELISA,ICC/IF,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes CCL27 / CTACK
Tested Reactivity Hu
Tested Application ELISA, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CCL27 / CTACK
Antigen Species Human
Immunogen E. coli derived recombinant Human CTACK / CCL27.
(FLLPPSTACC TQLYRKPLSD KLLRKVIQVE LQEADGDCHL QAFVLHLAQR SICIHPQNPS LSQWFEHQER KLHGTLPKLN FGMLRKMG)
Conjugation Un-conjugated
Alternate Names ILC; SCYA27; CTACK; Cutaneous T-cell-attracting chemokine; CTAK; PESKY; ESkine; Skinkine; ESKINE; Small-inducible cytokine A27; IL-11 R-alpha-locus chemokine; C-C motif chemokine 27; ALP; CC chemokine ILC

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66137 as a detection antibody
ICC/IF3.0 µg/ml
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10850 Human CCL27

Swiss-port # Q9Y4X3 Human C-C motif chemokine 27

Gene Symbol CCL27
Gene Full Name chemokine (C-C motif) ligand 27
Background This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene is chemotactic for skin-associated memory T lymphocytes. This cytokine may also play a role in mediating homing of lymphocytes to cutaneous sites. It specifically binds to chemokine receptor 10 (CCR10). Studies of a similar murine protein indicate that these protein-receptor interactions have a pivotal role in T cell-mediated skin inflammation. [provided by RefSeq, Sep 2014]
Function Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10. [UniProt]
Calculated MW 13 kDa

Images (3) Click the Picture to Zoom In

  • ARG66136 anti-CCL27 / CTACK antibody WB image

    Western blot: 250 - 0.24 ng of Human CTACK stained with ARG66136 anti-CCL27 / CTACK antibody, under reducing conditions.

  • ARG66136 anti-CCL27 / CTACK antibody standard curve image

    Sandwich ELISA: ARG66136 anti-CCL27 / CTACK antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66137 anti-CTACK / CCL27 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66136 anti-CCL27 / CTACK antibody WB image

    Western blot: 250 - 0.24 ng of Human CTACK stained with ARG66136 anti-CCL27 / CTACK antibody, under non-reducing conditions.