ARG56618

anti-CCL2 / MCP1 antibody

anti-CCL2 / MCP1 antibody for ELISA,IHC-Frozen sections,Neutralizing,Western blot and Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CCL2 / MCP1
Tested Reactivity Rat
Tested Application ELISA, IHC-Fr, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CCL2 / MCP1
Antigen Species Rat
Immunogen E.coli derived Recombinant Rat CCL2 / MCP1.
(QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTKLKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA PLNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN)
Conjugation Un-conjugated
Alternate Names MCP1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; Monocyte secretory protein JE; HSMCR30; Small-inducible cytokine A2; HC11; SMC-CF; GDCF-2; SCYA2; C-C motif chemokine 2; Monocyte chemoattractant protein 1; MCP-1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with (MCAF) as a detection antibody
IHC-Fr1.0 µg/ml
Neut4.0 - 6.0 µg/ml (To yield [ND50] of the biological activity of Rat MCP - 1(MCAF)(100 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24770 Rat CCL2

Swiss-port # P14844 Rat C-C motif chemokine 2

Gene Symbol Ccl2
Gene Full Name chemokine (C-C motif) ligand 2
Background This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
Function Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. [UniProt]
Highlight Related products:
MCP1 antibodies; MCP1 ELISA Kits; MCP1 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
Calculated MW 11 kDa
PTM Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

Images (3) Click the Picture to Zoom In

  • ARG56618 anti-CCL2 / MCP1 antibody WB image

    Western blot: 250 - 0.24 ng of Rat MCP1 stained with ARG56618 anti-CCL2 / MCP1 antibody, under reducing conditions.

  • ARG56618 anti-CCL2 / MCP1 antibody WB image

    Western blot: 250 - 0.24 ng of Rat MCP1 stained with ARG56618 anti-CCL2 / MCP1 antibody, under non-reducing conditions.

  • ARG56618 anti-CCL2 / MCP1 antibody standard curve image

    Sandwich ELISA: ARG56618 anti-CCL2 / MCP1 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56732 anti-CCL2 / MCP1 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.