ARG58363

anti-CA5A / Carbonic Anhydrase 5A antibody

anti-CA5A / Carbonic Anhydrase 5A antibody for ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CA5A / Carbonic Anhydrase 5A
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CA5A / Carbonic Anhydrase 5A
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 39-305 of Human CA5A (NP_001730.1).
Conjugation Un-conjugated
Alternate Names EC 4.2.1.1; Carbonic anhydrase VA; Carbonic anhydrase 5A, mitochondrial; GS1-21A4.1; CA5; CAV; CA5AD; Carbonate dehydratase VA; CAVA; CA-VA

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control LO2
Observed Size 35 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 763 Human CA5A

Swiss-port # P35218 Human Carbonic anhydrase 5A, mitochondrial

Gene Symbol CA5A
Gene Full Name carbonic anhydrase VA, mitochondrial
Background Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VA is localized in the mitochondria and expressed primarily in the liver. It may play an important role in ureagenesis and gluconeogenesis. CA5A gene maps to chromosome 16q24.3 and an unprocessed pseudogene has been assigned to 16p12-p11.2. [provided by RefSeq, Jul 2008]
Function Reversible hydration of carbon dioxide. Low activity. [UniProt]
Cellular Localization Mitochondrion,. [UniProt]
Calculated MW 35 kDa

Images (1) Click the Picture to Zoom In

  • ARG58363 anti-CA5A / Carbonic Anhydrase 5A antibody WB image

    Western blot: 25 µg of LO2 cell lysate stained with ARG58363 anti-CA5A / Carbonic Anhydrase 5A antibody at 1:1000 dilution.