ARG54834
anti-CA2 / Carbonic Anhydrase 2 antibody
anti-CA2 / Carbonic Anhydrase 2 antibody for ICC/IF,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CA2 / Carbonic Anhydrase 2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CA2 / Carbonic Anhydrase 2 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 54-83 (N-terminus) of Human CA2. |
| Conjugation | Un-conjugated |
| Alternate Names | Carbonic anhydrase C; EC 4.2.1.1; Car2; Carbonate dehydratase II; CA-II; Carbonic anhydrase II; CAC; HEL-76; Carbonic anhydrase 2; CAII |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse kidney |
Properties
| Form | Liquid |
|---|---|
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CA2 |
| Gene Full Name | carbonic anhydrase II |
| Background | The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014] |
| Function | Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6. [UniProt] |
| Cellular Localization | Cytoplasm. Cell membrane. Note=Colocalized with SLC26A6 at the surface of the cell membrane in order to form a bicarbonate transport metabolon. Displaced from the cytosolic surface of the cell membrane by PKC in phorbol myristate acetate (PMA)-induced cells |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 29 kDa |
Images (2) Click the Picture to Zoom In
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ARG54834 anti-CA2 / Carbonic Anhydrase 2 antibody ICC/IF image
Immunofluorescence: HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG54834 anti-CA2 / Carbonic Anhydrase 2 antibody (green) at 1:25 dilution, 1 h at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).
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ARG54834 anti-CA2 / Carbonic Anhydrase 2 antibody WB image
Western blot: 35 µg of Mouse kidney lysate stained with ARG54834 anti-CA2 / Carbonic Anhydrase 2 antibody at 1:1000 dilution.
