ARG64966
anti-CA1 / Carbonic Anhydrase 1 antibody
anti-CA1 / Carbonic Anhydrase 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CA1 / Carbonic Anhydrase 1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | IHC-P, WB |
| Specificity | Reported variants represent identical protein: NP_001729.1, NP_001122303.1, NP_001122302.1, NP_001122301.1. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CA1 / Carbonic Anhydrase 1 |
| Antigen Species | Human |
| Immunogen | C-QAIKTKGKRAP |
| Conjugation | Un-conjugated |
| Alternate Names | Carbonic anhydrase I; EC 4.2.1.1; Carbonate dehydratase I; Carbonic anhydrase B; Car1; HEL-S-11; CA-I; Carbonic anhydrase 1; CAB |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature. [provided by RefSeq, Sep 2009] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 29 kDa |
Images (2) Click the Picture to Zoom In
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ARG64966 anti-CA1 / Carbonic Anhydrase 1 antibody WB image
Western blot: Human Liver lysate (35 µg protein in RIPA buffer) stained with ARG64966 anti-CA1 / Carbonic Anhydrase 1 antibody at 0.03 µg/ml dilution.
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ARG64966 anti-CA1 / Carbonic Anhydrase 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64966 anti-CA1 / Carbonic Anhydrase 1 antibody at 2.5 µg/ml dilution followed by AP-staining.
