ARG44042
anti-Bnip 3L antibody
anti-Bnip 3L antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Bnip 3L |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Bnip 3L |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 95-180 of human BNIP3L (NP_004322.1). |
| Conjugation | Un-conjugated |
| Alternate Names | NIP3L; BNIP3a; Adenovirus E1B19K-binding protein B5; NIP3-like protein X; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A; NIX; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.01% Thimerosal and 50% Glycerol. |
| Preservative | 0.01% Thimerosal |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O60238 Human BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like Swiss-port # Q9Z2F7 Mouse BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like |
|---|---|
| Gene Symbol | BNIP3L |
| Gene Full Name | BCL2/adenovirus E1B 19kDa interacting protein 3-like |
| Background | A novel BH3 domain-containing protein was recently identified and designated Bnip3L, Bnip3 , or Nix. Bnip3L is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis. Bnip3L interacts with and overcomes suppression by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues. Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic-mitochondrial proteins. |
| Function | Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody |
| Calculated MW | 24 kDa |
| PTM | Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin. |
Images (4) Click the Picture to Zoom In
-
ARG44042 anti-Bnip 3L antibody IHC-P image
Immunohistochemistry: Mouse lung stained with ARG44042 anti-Bnip 3L antibody at 1:100 dilution.
-
ARG44042 anti-Bnip 3L antibody WB image
Western blot: Rat kidney stained with ARG44042 anti-Bnip 3L antibody at 1:1000 dilution.
-
ARG44042 anti-Bnip 3L antibody ICC/IF image
Immunofluorescence: NIH/3T3 stained with ARG44042 anti-Bnip 3L antibody at 1:100 dilution.
-
ARG44042 anti-Bnip 3L antibody IHC-P image
Immunohistochemistry: Rat kidney stained with ARG44042 anti-Bnip 3L antibody at 1:100 dilution.
