ARG56023
anti-Blood group A antigen antibody [HE-193]
anti-Blood group A antigen antibody [HE-193] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Mouse Monoclonal antibody [HE-193] recognizes Blood group A antigen |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | HE-193 |
| Isotype | IgM, kappa |
| Target Name | Blood group A antigen |
| Antigen Species | Human |
| Immunogen | A mixture of erythrocytes of blood group A and glycoprotein fraction isolated from the saliva of secretors with blood group A. |
| Conjugation | Un-conjugated |
| Alternate Names | Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; Fucosylglycoprotein 3-alpha-galactosyltransferase; GTB; Histo-blood group A transferase; NAGAT; A3GALT1; Histo-blood group ABO system transferase; EC 2.4.1.40; A transferase; Histo-blood group B transferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; A3GALNT; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; B transferase; EC 2.4.1.37 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | PEG precipitation |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P16442 Human Histo-blood group ABO system transferase |
|---|---|
| Gene Symbol | ABO |
| Gene Full Name | ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase) |
| Background | This gene encodes proteins related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. [UniProt] |
| Cellular Localization | Cell surface |
| Calculated MW | 41 kDa |
| PTM | The soluble form derives from the membrane form by proteolytic processing. |
Images (1) Click the Picture to Zoom In
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ARG56023 anti-Blood group A antigen antibody [HE-193] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human colon carcinoma stained with ARG56023 anti-Blood group A antigen antibody [HE-193].
