ARG58313
anti-Bik antibody
anti-Bik antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Bik |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Bik |
| Antigen Species | Human |
| Immunogen | Human Bik recombinant protein (Position: M1-R123). Human Bik shares 42.3% amino acid (aa) sequence identity with Mouse Bik. |
| Conjugation | Un-conjugated |
| Alternate Names | NBK; Bcl-2-interacting killer; BIP1; Apoptosis inducer NBK; BP4 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BIK |
| Gene Full Name | BCL2-interacting killer (apoptosis-inducing) |
| Background | The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins. [provided by RefSeq, Sep 2011] |
| Function | Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX. [UniProt] |
| Cellular Localization | Endomembrane system; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Around the nuclear envelope, and in cytoplasmic membranes. [UniProt] |
| Calculated MW | 18 kDa |
| PTM | Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage is a necessary step prior its degradation by the proteosome-dependent mechanism. [UniProt] |
Images (7) Click the Picture to Zoom In
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ARG58313 anti-Bik antibody FACS image
Flow Cytometry: MCF-7 cells were blocked with 10% normal goat serum, and then stained with ARG58313 anti-Bik antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG58313 anti-Bik antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse spleen tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58313 anti-Bik antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG58313 anti-Bik antibody WB image
Western blot: 50 µg of Rat spleen, Rat stomach, Rat intestine, MCF-7 whole cell lysate, A549 whole cell lysate and SKOV whole cell lysate stained with ARG58313 anti-Bik antibody at 0.5 µg/ml, overnight at 4°C, under reducing conditions.
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ARG58313 anti-Bik antibody FACS image
Flow Cytometry: THP-1 cells were blocked with 10% normal goat serum, and then stained with ARG58313 anti-Bik antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG58313 anti-Bik antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum, and then stained with ARG58313 anti-Bik antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG58313 anti-Bik antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat spleen tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58313 anti-Bik antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG58313 anti-Bik antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58313 anti-Bik antibody at 1 µg/ml dilution, overnight at 4°C.
