ARG64083
anti-Biglycan antibody
anti-Biglycan antibody for ICC/IF and Human,Mouse
Developmental Biology antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Biglycan |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Dog, Rat |
| Tested Application | ICC/IF |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Biglycan |
| Antigen Species | Human |
| Immunogen | C-DRLAIQFGNYKK (C-terminal) |
| Conjugation | Un-conjugated |
| Alternate Names | Biglycan; Bone/cartilage proteoglycan I; SLRR1A; PGI; DSPG1; PG-S1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein plays a role in assembly of collagen fibrils and muscle regeneration. It interacts with several proteins involved in muscular dystrophy, including alpha-dystroglycan, alpha- and gamma-sarcoglycan and collagen VI, and it is critical for the assembly of the dystrophin-associated protein complex. [provided by RefSeq, Nov 2009] |
| Research Area | Developmental Biology antibody; Signaling Transduction antibody |
| Calculated MW | 42 kDa |
| PTM | The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate. |
Images (2) Click the Picture to Zoom In
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ARG64083 anti-Biglycan antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed NIH/3T3 cells permeabilized with 0.15% Triton. Cells were stained with ARG64083 anti-Biglycan antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64083 anti-Biglycan antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton. Cells were stained with ARG64083 anti-Biglycan antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
