ARG65625
anti-Bid antibody
anti-Bid antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Bid |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Bid |
| Antigen Species | Human |
| Immunogen | Fusion protein of human Bid. |
| Conjugation | Un-conjugated |
| Alternate Names | p22 BID; BID; p15 BID; p11 BID; BH3-interacting domain death agonist; p13 BID; FP497 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 1.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P55957 Human BH3-interacting domain death agonist |
|---|---|
| Gene Symbol | BID |
| Gene Full Name | BH3 interacting domain death agonist |
| Background | This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. |
| Highlight | Related Antibody Duos and Panels: ARG30275 Pro-apoptotic Bcl2 protein Antibody Panel (BAX, BAK, Bid) Related products: Bid antibodies; Bid Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody |
| Calculated MW | 22 kDa |
| PTM | TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products. p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation. |
Images (2) Click the Picture to Zoom In
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ARG65625 anti-Bid antibody WB image
Western blot: 30 µg of Jurkat cell lysate stained with ARG65625 anti-Bid antibody at 1:500 dilution.
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ARG65625 anti-Bid antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human renal cancer tissue stained with ARG65625 anti-Bid antibody (left) at 1:25 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200)
Customer's Feedback
Excellent
Review for anti-Bid antibody
Application：WB
Sample：Jurkat
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
