ARG54027
anti-BiP / GRP78 antibody
anti-BiP / GRP78 antibody for ICC/IF,Western blot and Human,Rat
Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody
Publication5
Overview
| Product Description | Mouse Monoclonal antibody recognizes BiP / GRP78 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Target Name | BiP / GRP78 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human BiP/GRP78 protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | Heat shock 70 kDa protein 5; BiP; GRP-78; Endoplasmic reticulum lumenal Ca; MIF2; 78 kDa glucose-regulated protein; GRP78; Immunoglobulin heavy chain-binding protein; HEL-S-89n; BIP; 2+ |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Pressure cooking in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 0.8 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSPA5 |
| Gene Full Name | heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) |
| Background | Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER |
| Function | Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. [UniProt] |
| Cellular Localization | Endoplasmic reticulum lumen. Melanosome |
| Research Area | Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody |
| Calculated MW | 72 kDa |
Images (2) Click the Picture to Zoom In
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ARG54027 anti-BiP / GRP78 antibody WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG54027 anti-BiP / GRP78 antibody at 1:1000 dilution.
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ARG54027 anti-BiP / GRP78 antibody ICC/IF image
Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG54027 anti-BiP / GRP78 antibody at 1:50 dilution.
Customer's Feedback
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Review for anti-BiP / GRP78 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Endoplasmic reticulum stress promotes sorafenib resistance via miR-188-5p/hnRNPA2B1-mediated upregulation of PKM2 in hepatocellular carcinoma
WB / Human
Study of isolation and characterization of extracellular vesicles in senescent tumor cells induced by chemotherapeutics.
WB / Human
Melatonin Increases the Sensitivity of Hepatocellular Carcinoma to Sorafenib through the PERK-ATF4-Beclin1 Pathway
WB, ICC/IF / Human
Effects of calpain inhibitor on the apoptosis of hepatic stellate cells induced by calcium ionophore A23187.
WB / Human
Effects of calcium Ionophore A23187 on the apoptosis of hepatic stellate cells stimulated by transforming growth factor-β1.
WB / Rat
