ARG20530
anti-BiP / GRP78 antibody
anti-BiP / GRP78 antibody for ELISA,ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat,D. melanogaster ,Dog
Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes BiP / GRP78 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Dm, Dog |
| Predict Reactivity | Rat |
| Tested Application | ELISA, ICC/IF, IHC, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | BiP / GRP78 |
| Antigen Species | Human |
| Immunogen | Full length of Human BiP / GRP78 with His tagged at the N-terminus. |
| Conjugation | Un-conjugated |
| Alternate Names | Heat shock 70 kDa protein 5; BiP; GRP-78; Endoplasmic reticulum lumenal Ca; MIF2; 78 kDa glucose-regulated protein; GRP78; Immunoglobulin heavy chain-binding protein; HEL-S-89n; BIP; 2+ |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSPA5 |
| Gene Full Name | heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) |
| Background | The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010] |
| Function | Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. [UniProt] |
| Research Area | Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody |
| Calculated MW | 72 kDa |
Images (2) Click the Picture to Zoom In
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ARG20530 anti-BiP / GRP78 antibody ICC/IF image
Immunofluorescence: Heat Shocked (42°C for 1 hour) HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ARG20530 anti-BiP / GRP78 antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain. Middle: ARG20530 anti-BiP / GRP78 antibody. Right: Composite.
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ARG20530 anti-BiP / GRP78 antibody WB image
Western blot: Glia GD cell lysates stained with ARG20530 anti-BiP / GRP78 antibody at 1:1000 dilution.
