ARG58688

anti-Beclin 1 antibody

anti-Beclin 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Autophagy Study antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Beclin 1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Beclin 1
Antigen Species Human
Immunogen Synthetic peptide derived from Human Beclin 1.
Conjugation Un-conjugated
Alternate Names BECN1; Beclin 1; VPS30; ATG6; Coiled-Coil Myosin-Like BCL2-Interacting Protein; Beclin 1, Autophagy Related; Beclin-1; Beclin 1 (Coiled-Coil, Moesin-Like BCL2 Interacting Protein); Beclin 1 (Coiled-Coil, Moesin-Like BCL2-Interacting Protein); ATG6 Autophagy Related 6 Homolog (S. Cerevisiae); Testis Secretory Sperm-Binding Protein Li 215e; ATG6 Autophagy Related 6 Homolog; Beclin1-Gamma; Protein GT197; Beclin1-Beta; Beclin1; GT197

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HEK293
Observed Size ~ 52 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 114558 Rat BECN1

GeneID: 56208 Mouse BECN1

GeneID: 8678 Human BECN1

Gene Symbol BECN1
Gene Full Name beclin 1, autophagy related
Background This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Function Protects against infection by a neurovirulent strain of Sindbis virus. May play a role in antiviral host defense. [UniProt]
Cellular Localization Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Mitochondrion, Nucleus. [UniProt]
Highlight Related products:
Beclin 1 antibodies; Beclin 1 Duos / Panels;
Research Area Cancer antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Autophagy Study antibody
Calculated MW 52 kDa
PTM Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG58688 anti-Beclin 1 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG58688 anti-Beclin 1 antibody (green). Nuclear staining (blue).

  • ARG58688 anti-Beclin 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast cancer tissue stained with ARG58688 anti-Beclin 1 antibody.

  • ARG58688 anti-Beclin 1 antibody WB image

    Western blot: HEK293 cell lysate stained with ARG58688 anti-Beclin 1 antibody.