ARG54094
anti-Beclin 1 antibody
anti-Beclin 1 antibody for Western blot and Human,Mouse,Rat,Hamster,Monkey
Cancer antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Autophagy Study antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody recognizes Beclin 1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Hm, Mk |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | Beclin 1 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human Beclin 1 protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | BECN1; Beclin 1; VPS30; ATG6; Coiled-Coil Myosin-Like BCL2-Interacting Protein; Beclin 1, Autophagy Related; Beclin-1; Beclin 1 (Coiled-Coil, Moesin-Like BCL2 Interacting Protein); Beclin 1 (Coiled-Coil, Moesin-Like BCL2-Interacting Protein); ATG6 Autophagy Related 6 Homolog (S. Cerevisiae); Testis Secretory Sperm-Binding Protein Li 215e; ATG6 Autophagy Related 6 Homolog; Beclin1-Gamma; Protein GT197; Beclin1-Beta; Beclin1; GT197 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | 55 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| Preservative | 0.2% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | Lot dependent |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BECN1 |
| Gene Full Name | beclin 1, autophagy related |
| Background | This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| Function | Protects against infection by a neurovirulent strain of Sindbis virus. May play a role in antiviral host defense. [UniProt] |
| Cellular Localization | Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Mitochondrion, Nucleus. [UniProt] |
| Highlight | Related products: Beclin 1 antibodies; Beclin 1 Duos / Panels; |
| Research Area | Cancer antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Autophagy Study antibody |
| Calculated MW | 52 kDa |
| PTM | Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG54094 anti-Beclin 1 antibody WB image
Western blot: 20 µg of HeLa cell lysate stained with ARG54094 anti-Beclin 1 antibody at 1:1000 dilution.
Customer's Feedback
Excellent
Review for anti-Beclin 1 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-Beclin 1 antibody
Application：WB
Sample：Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-Beclin 1 antibody
Application：WB
Sample：Mouse brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Dexmedetomidine alleviates LPS-induced apoptosis and inflammation in macrophages by eliminating damaged mitochondria via PINK1 mediated mitophagy.
WB / Mouse
