ARG51569
anti-Bcl XL phospho (Ser62) antibody
anti-Bcl XL phospho (Ser62) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Bcl XL phospho (Ser62) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Bcl XL |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 62(A-D-S(p)-P-A) derived from Human BCL-XL . |
| Conjugation | Un-conjugated |
| Alternate Names | Apoptosis regulator Bcl-X; BCLXS; BCL-XL/S; PPP1R52; bcl-xS; Bcl-2-like protein 1; Bcl2-L-1; Bcl-X; BCLX; bcl-xL; BCL2L; BCLXL |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BCL2L1 |
| Gene Full Name | BCL2-like 1 |
| Background | Potent inhibitor of cell death. Inhibits activation of caspases . Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(S) promotes apoptosis |
| Function | Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. Isoform Bcl-X(S) promotes apoptosis. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30233 Phospho Bcl XL Antibody Duo (Total, pS62) Related products: Bcl XL antibodies; Bcl XL Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 26 kDa |
| PTM | Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis. |
Images (4) Click the Picture to Zoom In
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ARG51569 anti-Bcl XL phospho (Ser62) antibody WB image
Western blot: Extracts from HeLa cells untreated or treated with EGF stained with ARG51569 anti-Bcl XL phospho (Ser62) antibody.
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ARG51569 anti-Bcl XL phospho (Ser62) antibody WB image
Western blot: Extracts from HeLa cells, treated with Noc or calf intestinal phosphatase (CIP), stained with ARG51569 anti-Bcl XL phospho (Ser62) antibody.
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ARG51569 anti-Bcl XL phospho (Ser62) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51569 anti-Bcl XL phospho (Ser62) antibody (red).
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ARG51569 anti-Bcl XL phospho (Ser62) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51569 anti-Bcl XL phospho (Ser62) antibody.
