ARG58302
anti-Bcl 2L2 / Bcl W antibody
anti-Bcl 2L2 / Bcl W antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Bcl 2L2 / Bcl W |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Hm |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Bcl 2L2 / Bcl W |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to a sequence in the middle region of Human BCL2L2(74-88aa SAQQRFTQVSDELFQ), identical to the related Rat and Mouse sequences. |
| Conjugation | Un-conjugated |
| Alternate Names | PPP1R51; Bcl-2-like protein 2; Bcl2-L-2; BCLW; Apoptosis regulator Bcl-W; BCL-W; BCL2-L-2 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BCL2L2 |
| Gene Full Name | BCL2-like 2 |
| Background | This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene. [provided by RefSeq, Dec 2010] |
| Function | Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX. [UniProt] |
| Cellular Localization | Mitochondrion membrane; Peripheral membrane protein. Loosely associated with the mitochondrial membrane in healthy cells. During apoptosis, tightly bound to the membrane. [UniProt] |
| Calculated MW | 21 kDa |
Images (3) Click the Picture to Zoom In
-
ARG58302 anti-Bcl 2L2 / Bcl W antibody FACS image
Flow Cytometry: PC-3 cells were blocked with 10% normal goat serum, and then stained with ARG58302 anti-Bcl 2L2 / Bcl W antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
-
ARG58302 anti-Bcl 2L2 / Bcl W antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human rectal cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58302 anti-Bcl 2L2 / Bcl W antibody at 1 µg/ml dilution, overnight at 4°C.
-
ARG58302 anti-Bcl 2L2 / Bcl W antibody WB image
Western blot: 50 µg of HeLa whole cell lysate stained with ARG58302 anti-Bcl 2L2 / Bcl W antibody at 0.5 µg/ml, overnight at 4°C, under reducing conditions.
