ARG23555
anti-Bacillus anthracis protective antigen antibody [C3]
anti-Bacillus anthracis protective antigen antibody [C3] for ELISA,Western blot and Bacteria
Overview
| Product Description | Mouse Monoclonal antibody [C3] recognizes Bacillus anthracis protective antigen. Mouse anti Anthrax Protective Antigen antibody, clone C3 recognizes the Protective Antigen of the anthrax toxin. Bacillus anthracis produces a toxin composed of three proteins, the Protective Antigen (PA), the Edema Factor (EF) and the Lethal Factor (LF). The three proteins depend on each other for their toxic effect. Both EF and LF functions intracellularly but are too large to enter the cell through existing channels. The PA is responsible for transporting the EF and LF into cells. The 83kDa PA (PA83) binds to the cell surface where a 20 kDa N-terminal fragment (PA20) is cleaved off, exposing binding sites for LF and EF. The remaining 63 kDa portion (PA63) oligomerizes to form a heptamer pre-pore, which then associates with up to three molecules of EF and/or LF. The receptor complex is internalized within endocytotic vessels that are subsequently acidified, transforming the PA heptamer into a pore that releases the active enzymes EF (an adenylate cyclase) and LF (a protease targeting MAPKKs) into the cytosol. Mouse anti Anthrax Protective Antigen antibody, clone C3 does not cross-react with the Lethal Factor of Bacillus anthracis, Yersinia pestis, Francisella tularensis or Toxoplasma gondii. The antibody recognizes both the 83kDa and 63kDa truncated form of PA. |
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| Tested Reactivity | Bacteria |
| Tested Application | ELISA, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | C3 |
| Isotype | IgG1 |
| Target Name | Bacillus anthracis protective antigen |
| Antigen Species | Bacteria |
| Immunogen | Highly purified protective antigen of Bacillus anthracis. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
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| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
