ARG66116

anti-BTC / Betacellulin antibody

anti-BTC / Betacellulin antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes BTC / Betacellulin
Tested Reactivity Hu
Tested Application ELISA, IHC-P, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name BTC / Betacellulin
Antigen Species Human
Immunogen E. coli derived recombinant Human Betacellulin.
(DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY)
Conjugation Un-conjugated
Alternate Names Probetacellulin; BTC

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66117 as a detection antibody
IHC-P2.5 µg/ml
Neut≤ 0.1 µg/ml (To yield [ND50] of the biological activity of Human Betacellulin (0.1 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 685 Human BTC

Swiss-port # P35070 Human Probetacellulin

Gene Symbol BTC
Gene Full Name betacellulin
Background The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. [provided by RefSeq, Jul 2008]
Function Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. [UniProt]
Calculated MW 20 kDa

Images (3) Click the Picture to Zoom In

  • ARG66116 anti-BTC / Betacellulin antibody WB image

    Western blot: 250 - 0.24 ng of Human Betacellulin stained with ARG66116 anti-BTC / Betacellulin antibody, under non-reducing conditions.

  • ARG66116 anti-BTC / Betacellulin antibody standard curve image

    Sandwich ELISA: ARG66116 anti-BTC / Betacellulin antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66117 anti-Betacellulin antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66116 anti-BTC / Betacellulin antibody WB image

    Western blot: 250 - 0.24 ng of Human Betacellulin stained with ARG66116 anti-BTC / Betacellulin antibody, under reducing conditions.