Overview

Product Description	Rabbit Polyclonal antibody recognizes BPGM
Tested Reactivity	Hu, Ms, Rat
Tested Application	ICC/IF, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	BPGM
Antigen Species	Human
Immunogen	Recombinant Protein of Human BPGM.
Conjugation	Un-conjugated
Alternate Names	2,3-diphosphoglycerate mutase; BPGM; Bisphosphoglycerate mutase; DPGM; EC 5.4.2.11; 2,3-bisphosphoglycerate synthase; EC 5.4.2.4; BPG-dependent PGAM; EC 3.1.3.13; 2,3-bisphosphoglycerate mutase, erythrocyte

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	Mouse spleen

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 12183 Mouse BPGM GeneID: 669 Human BPGM Swiss-port # P07738 Human Bisphosphoglycerate mutase Swiss-port # P15327 Mouse Bisphosphoglycerate mutase
Gene Symbol	BPGM
Gene Full Name	2,3-bisphosphoglycerate mutase
Background	2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009]
Function	Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) and phosphatase (EC 3.1.3.13) activities. [UniProt]
Calculated MW	30 kDa
PTM	Glycation of Lys-159 in diabetic patients inactivates the enzyme.

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