ARG41280

anti-BLZF1 antibody

anti-BLZF1 antibody for ICC/IF,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes BLZF1
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name BLZF1
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-400 of Human BLZF1 (NP_003657.1).
Conjugation Un-conjugated
Alternate Names JEM1; Golgin-45; JEM-1; p45 basic leucine-zipper nuclear factor; Basic leucine zipper nuclear factor 1; JEM-1s; GOLGIN-45

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse skeletal muscle
Observed Size 50 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 66352 Mouse BLZF1

GeneID: 8548 Human BLZF1

Swiss-port # Q8R2X8 Mouse Golgin-45

Swiss-port # Q9H2G9 Human Golgin-45

Gene Symbol BLZF1
Gene Full Name basic leucine zipper nuclear factor 1
Function Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface. [UniProt]
Cellular Localization Golgi apparatus lumen. Isoform 1: Nucleus. Isoform 2: Cytoplasm. [UniProt]
Calculated MW 45 kDa
PTM ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.

Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG41280 anti-BLZF1 antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG41280 anti-BLZF1 antibody.

  • ARG41280 anti-BLZF1 antibody WB image

    Western blot: 25 ug of Mouse skeletal muscle lysate stained with ARG41280 anti-BLZF1 antibody at 1:1000 dilution.