ARG41883
anti-BIRC3 / cIAP2 antibody
anti-BIRC3 / cIAP2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes BIRC3 / cIAP2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BIRC3 / cIAP2 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-180 of Human BIRC3 / cIAP2. (NP_001156.1) |
| Conjugation | Un-conjugated |
| Alternate Names | MIHC; EC 6.3.2.-; hIAP1; API2; HIAP1; Baculoviral IAP repeat-containing protein 3; Inhibitor of apoptosis protein 1; hIAP-1; IAP homolog C; CIAP2; RNF49; C-IAP2; IAP-1; RING finger protein 49; HAIP1; c-IAP2; TNFR2-TRAF-signaling complex protein 1; Apoptosis inhibitor 2; MALT2; AIP1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse lung | ||||||||
| Observed Size | ~ 80 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O08863 Mouse Baculoviral IAP repeat-containing protein 3 Swiss-port # Q13489 Human Baculoviral IAP repeat-containing protein 3 |
|---|---|
| Gene Symbol | BIRC3 |
| Gene Full Name | baculoviral IAP repeat containing 3 |
| Background | This gene encodes a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified. [provided by RefSeq, Aug 2011] |
| Function | Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, IKBKE, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. [UniProt] |
| Calculated MW | 68 kDa |
| PTM | Auto-ubiquitinated and degraded by the proteasome in apoptotic cells. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG41883 anti-BIRC3 / cIAP2 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG41883 anti-BIRC3 / cIAP2 antibody at 1:100 dilution.
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ARG41883 anti-BIRC3 / cIAP2 antibody WB image
Western blot: 25 µg of Mouse lung lysate stained with ARG41883 anti-BIRC3 / cIAP2 antibody at 1:1000 dilution.
