ARG64302
anti-BHMT antibody
anti-BHMT antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes BHMT |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BHMT |
| Antigen Species | Human |
| Immunogen | C-EQQLKELFEKQK |
| Conjugation | Un-conjugated |
| Alternate Names | HEL-S-61p; Betaine--homocysteine S-methyltransferase 1; EC 2.1.1.5; BHMT1 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008] |
| Research Area | Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 45 kDa |
Images (4) Click the Picture to Zoom In
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ARG64302 anti-BHMT antibody WB image
Western Blot: Rat Liver lysate (35 µg protein in RIPA buffer) stained with ARG64302 anti-BHMT antibody at 0.03 µg/ml dilution.
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ARG64302 anti-BHMT antibody WB image
Western Blot: 1). Mock transfection; 2) BHMT RC203148) expressing plasmid transfected HEK293 cell lysate standed with ARG64302 anti-BHMT antibody
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ARG64302 anti-BHMT antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64302 anti-BHMT antibody at 5 µg/ml dilution followed by AP-staining.
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ARG64302 anti-BHMT antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64302 anti-BHMT antibody at 5 µg/ml dilution followed by AP-staining.
