ARG56932
anti-BHMT antibody [3D6]
anti-BHMT antibody [3D6] for Flow cytometry,ICC/IF,Western blot and Human,Mouse
Overview
| Product Description | Mouse Monoclonal antibody [3D6] recognizes BHMT |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3D6 |
| Isotype | IgG2a, kappa |
| Target Name | BHMT |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-406 of Human BHMT. |
| Conjugation | Un-conjugated |
| Alternate Names | HEL-S-61p; Betaine--homocysteine S-methyltransferase 1; EC 2.1.1.5; BHMT1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O35490 Mouse Betaine--homocysteine S-methyltransferase 1 Swiss-port # Q93088 Human Betaine--homocysteine S-methyltransferase 1 |
|---|---|
| Gene Symbol | BHMT |
| Gene Full Name | betaine--homocysteine S-methyltransferase |
| Background | This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008] |
| Function | Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline. [UniProt] |
| Calculated MW | 45 kDa |
Images (3) Click the Picture to Zoom In
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ARG56932 anti-BHMT antibody [3D6] ICC/IF image
Immunoflorescense: Hep3B cell line stained with ARG56932 anti-BHMT antibody [3D6] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG56932 anti-BHMT antibody [3D6] FACS image
Flow Cytometry: Hep3B cell line stained with ARG56932 anti-BHMT antibody [3D6] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.
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ARG56932 anti-BHMT antibody [3D6] WB image
Western blot: 20 µg of Mouse liver stained with ARG56932 anti-BHMT antibody [3D6] at 1:1000.
