ARG63290
anti-BERP / RNF22 antibody
anti-BERP / RNF22 antibody for Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes BERP / RNF22 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Dog |
| Tested Application | WB |
| Specificity | Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BERP / RNF22 |
| Antigen Species | Human |
| Immunogen | AKREDSPGPEVQP-C |
| Conjugation | Un-conjugated |
| Alternate Names | BERP; RING finger protein 97; RNF97; HAC1; Brain-expressed RING finger protein; Tripartite motif-containing protein 3; RING finger protein 22; RNF22 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 81 kDa |
Images (3) Click the Picture to Zoom In
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ARG63290 anti-BERP / RNF22 antibody WB image
Western Blot: Mouse Brain lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63290 anti-BERP / RNF22 antibody at 0.05 µg/ml dilution.
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ARG63290 anti-BERP / RNF22 antibody WB image
Western Blot: 1). Mock transfection; 2) TRIM3 (RC211928) expressing plasmid transfected HEK293 cell lysate standed with ARG63290 anti-BERP / RNF22 antibody
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ARG63290 anti-BERP / RNF22 antibody WB image
Western blot: 35 µg of Human cerebellum (A) and Rat brain (B) lysates (in RIPA buffer) stained with ARG63290 anti-BERP / RNF22 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
