ARG63536

anti-BAIAP2 antibody

anti-BAIAP2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Neuroscience antibody

Overview

Product Description Goat Polyclonal antibody recognizes BAIAP2
Tested Reactivity Hu
Predict Reactivity Ms, Rat
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise only human isoform 1 according to NP_059344.1.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name BAIAP2
Antigen Species Human
Immunogen C-SGSGTLVSTV
Conjugation Un-conjugated
Alternate Names IRS-58; Fas ligand-associated factor 3; Brain-specific angiogenesis inhibitor 1-associated protein 2; Insulin receptor substrate p53; IRSp53; BAI-associated protein 2; IRSP53; FLAF3; IRSp53/58; BAI1-associated protein 2; Insulin receptor substrate protein of 53 kDa; BAP2; Protein BAP2; Insulin receptor substrate p53/p58

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10458 Human BAIAP2

Swiss-port # Q9UQB8 Human Brain-specific angiogenesis inhibitor 1-associated protein 2

Background The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]
Research Area Neuroscience antibody
Calculated MW 61 kDa
PTM Phosphorylated on tyrosine residues by INSR in response to insulin treatment.

Images (2) Click the Picture to Zoom In

  • ARG63536 anti-BAIAP2 antibody WB image

    Western Blot: Human Brain lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63536 anti-BAIAP2 (isoform 1) antibody at 1 µg/ml dilution.

  • ARG63536 anti-BAIAP2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63536 anti-BAIAP2 antibody at 5 µg/ml dilution followed by AP-staining.