ARG54369
anti-BAFF antibody
anti-BAFF antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes BAFF |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | This antibody recognizes human, mouse, and rat BAFF (approx. 38kDa). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BAFF |
| Antigen Species | Human |
| Immunogen | Peptide corresponding to aa 254-269 at the C-terminus of human BAFF. |
| Conjugation | Un-conjugated |
| Alternate Names | BLYS; TALL1; Dendritic cell-derived TNF-like molecule; THANK; DTL; TNF- and APOL-related leukocyte expressed ligand 1; TNFSF20; CD257; BLyS; Tumor necrosis factor ligand superfamily member 13B; ZTNF4; CD antigen CD257; B lymphocyte stimulator; TALL-1; BAFF; B-cell-activating factor |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HL60 |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunoaffinity chroma-tography |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9WU72 Mouse Tumor necrosis factor ligand superfamily member 13B Swiss-port # Q9Y275 Human Tumor necrosis factor ligand superfamily member 13B |
|---|---|
| Gene Symbol | TNFSF13B |
| Gene Full Name | tumor necrosis factor (ligand) superfamily, member 13b |
| Background | A novel member of the TNF superfamily was recently identified and designated BAFF (B Cell Activating Factor of the TNF Family) or BlyS (B Lymphocyte Stimulator), TALL-1 (TNF- and ApoL-Related Leukocyte-Expressed Ligand), and THANK (TNF Homologue that Activates Apoptosis, NF- B and c-jun N-terminal Kinase). BAFF is designated a B cell stimulator since it induces B cell proliferation and immunoglobulin secretion. BAFF and its receptors are involved in the development of systemic lupus erythematosus and other B cell-associated autoimmune diseases. |
| Function | Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity. Isoform 3: Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody |
| Calculated MW | 31 kDa |
| PTM | The soluble form derives from the membrane form by proteolytic processing. Isoform 2 is not efficiently shed from the membrane unlike isoform 1. N-glycosylated. |
Images (2) Click the Picture to Zoom In
-
ARG54369 anti-BAFF antibody IHC image
Immunohistochemistry: Human spleen stained with ARG54369 anti-BAFF antibody at 20 μg/ml dilution.
-
ARG54369 anti-BAFF antibody WB image
Western Blot: H:HL60; M: murine spleen tissue lysate stained with ARG54369 anti-BAFF antibody at 1 μg/ml dilution.
