Overview

Product Description	Rabbit Polyclonal antibody recognizes BACE1
Tested Reactivity	Hu, Ms, Rat
Tested Application	IP, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	BACE1
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human BACE1.
Conjugation	Un-conjugated
Alternate Names	Beta-site APP cleaving enzyme 1; BACE; Beta-secretase 1; Membrane-associated aspartic protease 2; HSPC104; Memapsin-2; ASP2; Asp 2; EC 3.4.23.46; Aspartyl protease 2; Beta-site amyloid precursor protein cleaving enzyme 1

Application Instructions

Application Suggestion	Tested Application Dilution IP 1:40 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	SH-SY5Y

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 23621 Human BACE1 GeneID: 23821 Mouse BACE1 GeneID: 29392 Rat BACE1 Swiss-port # P56817 Human Beta-secretase 1 Swiss-port # P56818 Mouse Beta-secretase 1 Swiss-port # P56819 Rat Beta-secretase 1 More
Gene Symbol	BACE1
Gene Full Name	beta-site APP-cleaving enzyme 1
Background	Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]
Function	Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. [UniProt]
Cellular Localization	Membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Cytoplasmic vesicle membrane. Membrane raft. Note=Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. [UniProt]
Calculated MW	56 kDa
PTM	N-Glycosylated. Acetylated in the endoplasmic reticulum at Lys-126, Lys-275, Lys-279, Lys-285, Lys-299, Lys-300 and Lys-307. Acetylation by NAT8 and NAT8B is transient and deacetylation probably occurs in the Golgi. Acetylation regulates the maturation, the transport to the plasma membrane, the stability and the expression of the protein. Palmitoylation mediates lipid raft localization. [UniProt]

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