ARG54698

anti-B Raf antibody

anti-B Raf antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes B Raf
Tested Reactivity Hu
Predict Reactivity Ms, Chk
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name B Raf
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 424-453 of Human B Raf (NP_004324.2).
Conjugation Un-conjugated
Alternate Names BRAF1; v-Raf murine sarcoma viral oncogene homolog B1; Proto-oncogene B-Raf; p94; Serine/threonine-protein kinase B-raf; RAFB1; EC 2.7.11.1; B-RAF1; NS7

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
ICC/IF1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Purification Protein A purified
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 673 Human BRAF

Swiss-port # P15056 Human Serine/threonine-protein kinase B-raf

Gene Symbol BRAF
Gene Full Name B-Raf proto-oncogene, serine/threonine kinase
Background This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
Function Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. [From Uniprot]
Cellular Localization Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
Calculated MW 84 kDa
PTM Phosphorylation at Ser-365 by SGK1 inhibits its activity.
Methylation at Arg-671 decreases stability and kinase activity.
Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.

Images (4) Click the Picture to Zoom In

  • ARG54698 anti-B Raf antibody WB image

    Western blot: 30 µg of U87 cell lysate stained with ARG54698 anti-B Raf antibody at 1:500 dilution.

  • ARG54698 anti-B Raf antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG54698 anti-B Raf antibody. DAPI (blue) for nuclear staining.

  • ARG54698 anti-B Raf antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG54698 anti-B Raf antibody.

  • ARG54698 anti-B Raf antibody FACS image

    Flow Cytometry: HeLa cells stained with ARG54698 anti-B Raf antibody (right histogram) or without primary antibody control (left histogram).

Customer's Feedback

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Review for anti-B Raf antibody

Application:WB

Sample:U87

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC