ARG54673
anti-B Raf antibody
anti-B Raf antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes B Raf |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, ICC/IF, IHC-P, WB |
| Specificity | At least two isoforms of B-raf are known to exist; this antibody will detect both isoforms. This antibody will not cross-react with C-raf. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | B Raf |
| Immunogen | Synthetic peptide (19 aa) within aa. 330-380 Human B-raf protein. |
| Conjugation | Un-conjugated |
| Alternate Names | BRAF1; v-Raf murine sarcoma viral oncogene homolog B1; Proto-oncogene B-Raf; p94; Serine/threonine-protein kinase B-raf; RAFB1; EC 2.7.11.1; B-RAF1; NS7 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | Human Brain Tissue Lysate | ||||||||||
| Observed Size | 99 kDa. Multiple post-translational modifications, commonly observed at 95-100 kDa. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P15056 Human Serine/threonine-protein kinase B-raf Swiss-port # P28028 Mouse Serine/threonine-protein kinase B-raf |
|---|---|
| Gene Symbol | BRAF |
| Gene Full Name | B-Raf proto-oncogene, serine/threonine kinase |
| Background | B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance. | |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody |
| Calculated MW | 84 kDa |
| PTM | Phosphorylation at Ser-365 by SGK1 inhibits its activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF. |
Images (3) Click the Picture to Zoom In
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ARG54673 anti-B Raf antibody ICC/IF image
Immunofluorescence: Human small intestine tissue stained with ARG54673 anti-B Raf antibody at 20 μg/ml.
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ARG54673 anti-B Raf antibody IHC image
Immunohistochemistry: Human small intestine tissue stained with ARG54673 anti-B Raf antibody at 2.5 μg/ml.
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ARG54673 anti-B Raf antibody WB image
Western blot: Human brain tissue lysate stained with ARG54673 anti-B Raf antibody at (A) 1 and (B) 2 μg/ml.
