ARG10555
anti-B Raf (V600E) antibody [K21-F] (FITC)
anti-B Raf (V600E) antibody [K21-F] (FITC) for Flow cytometry and Human
Overview
| Product Description | FITC-conjugated Rabbit Monoclonal antibody [K21-F] recognizes B Raf (V600E) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | K21-F |
| Isotype | IgG |
| Target Name | B Raf (V600E) |
| Antigen Species | Human |
| Conjugation | FITC |
| Alternate Names | BRAF1; v-Raf murine sarcoma viral oncogene homolog B1; Proto-oncogene B-Raf; p94; Serine/threonine-protein kinase B-raf; RAFB1; EC 2.7.11.1; B-RAF1; NS7 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | Aqueous buffer solution, 0.05% Sodium azide and 10 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 10 mg/ml BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P15056 Human Serine/threonine-protein kinase B-raf |
|---|---|
| Gene Symbol | BRAF |
| Gene Full Name | B-Raf proto-oncogene, serine/threonine kinase |
| Background | This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008] |
| Function | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. [UniProt] |
| Calculated MW | 84 kDa |
| PTM | Phosphorylation at Ser-365 by SGK1 inhibits its activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF. |
Images (2) Click the Picture to Zoom In
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ARG10555 anti-B Raf (V600E) antibody [K21-F] (FITC) FACS image
Flow Cytometry: HT29 cells were fixed, permeabilized and stained with ARG10555 anti-B Raf (V600E) antibody [K21-F] (FITC) (green, 10 µl for HT29 per test) or with an isotype control (black).
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ARG10555 anti-B Raf (V600E) antibody [K21-F] (FITC) FACS image
Flow Cytometry: Chronic lymphocytic leukemia cells were fixed, permeabilized and stained with ARG10555 anti-B Raf (V600E) antibody [K21-F] (FITC) (blue, 4 µl for lymphocytes per test) or with an isotype control (black).
