ARG64412
anti-Aspartate Aminotransferase antibody
anti-Aspartate Aminotransferase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Aspartate Aminotransferase |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Rat |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Aspartate Aminotransferase |
| Antigen Species | Human |
| Immunogen | C-RSYRYWDAEKR |
| Conjugation | Un-conjugated |
| Alternate Names | Cysteine transaminase, cytoplasmic; cAspAT; GIG18; Glutamate oxaloacetate transaminase 1; cCAT; EC 2.6.1.3; Cysteine aminotransferase, cytoplasmic; ASTQTL1; AST1; EC 2.6.1.1; Transaminase A; Aspartate aminotransferase, cytoplasmic |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P17174 Human Aspartate aminotransferase, cytoplasmic |
|---|---|
| Background | Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 46 kDa |
Images (3) Click the Picture to Zoom In
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ARG64412 anti-Aspartate Aminotransferase antibody WB image
Western blot: 35 µg of Human Liver lysate stained with ARG64412 anti-Aspartate Aminotransferase antibody at 0.01 µg/ml dilution.
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ARG64412 anti-Aspartate Aminotransferase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64412 anti-Aspartate Aminotransferase antibody at 5 µg/ml dilution followed by AP-staining.
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ARG64412 anti-Aspartate Aminotransferase antibody IHC image
Immunohistochemistry: paraffin-embedded Human Heart (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64412 anti-Aspartate Aminotransferase antibody at 2.5 µg/ml dilution, followed by AP-staining.
