ARG57174
anti-Aspartate Aminotransferase antibody [3G11]
anti-Aspartate Aminotransferase antibody [3G11] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [3G11] recognizes Aspartate Aminotransferase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3G11 |
| Isotype | IgG2a, kappa |
| Target Name | Aspartate Aminotransferase |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-413 of Human Aspartate Aminotransferase |
| Conjugation | Un-conjugated |
| Alternate Names | Cysteine transaminase, cytoplasmic; cAspAT; GIG18; Glutamate oxaloacetate transaminase 1; cCAT; EC 2.6.1.3; Cysteine aminotransferase, cytoplasmic; ASTQTL1; AST1; EC 2.6.1.1; Transaminase A; Aspartate aminotransferase, cytoplasmic |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P17174 Human Aspartate aminotransferase, cytoplasmic |
|---|---|
| Gene Symbol | GOT1 |
| Gene Full Name | glutamic-oxaloacetic transaminase 1, soluble |
| Background | Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008] |
| Function | Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. [UniProt] |
| Calculated MW | 46 kDa |
Images (5) Click the Picture to Zoom In
-
ARG57174 anti-Aspartate Aminotransferase antibody [3G11] FACS image
Flow Cytometry: Hep3B cell line stained with ARG57174 anti-Aspartate Aminotransferase antibody [3G11] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
-
ARG57174 anti-Aspartate Aminotransferase antibody [3G11] WB image
Western blot: 40 µg of A427 cell lysate stained with ARG57174 anti-Aspartate Aminotransferase antibody [3G11] at 1:1000.
-
ARG57174 anti-Aspartate Aminotransferase antibody [3G11] FACS image
Flow Cytometry: HeLa cell line stained with ARG57174 anti-Aspartate Aminotransferase antibody [3G11] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
-
ARG57174 anti-Aspartate Aminotransferase antibody [3G11] WB image
Western blot: 100 ng of 1) Recombinant Human GOT1 protein, and 2) Recombinant Human GOT2 protein stained with ARG57174 anti-Aspartate Aminotransferase antibody [3G11] at 1:1000.
-
ARG57174 anti-Aspartate Aminotransferase antibody [3G11] WB image
Western blot: 10 µg of 1) 293T, and 2) GOT1 Transfected 293T cell lysate stained with ARG57174 anti-Aspartate Aminotransferase antibody [3G11] at 1:1000.
