ARG64392
anti-Arylsulfatase B antibody
anti-Arylsulfatase B antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Death antibody; Controls and Markers antibody; Metabolism antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Arylsulfatase B |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_000037.2; NP_942002.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Arylsulfatase B |
| Antigen Species | Human |
| Immunogen | C-KLARGHTNGTKPLD |
| Conjugation | Un-conjugated |
| Alternate Names | N-acetylgalactosamine-4-sulfatase; EC 3.1.6.12; MPS6; ASB; G4S; Arylsulfatase B |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Cell Death antibody; Controls and Markers antibody; Metabolism antibody |
| Calculated MW | 60 kDa |
| PTM | The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD). |
Images (2) Click the Picture to Zoom In
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ARG64392 anti-Arylsulfatase B antibody WB image
Western Blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG64392 anti-ARSB antibody at 0.03 µg/ml dilution.
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ARG64392 anti-Arylsulfatase B antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Skeletal Muscle. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64392 anti-ARSB antibody at 5 µg/ml dilution followed by AP-staining.
