ARG59661

anti-Annexin A8 antibody

anti-Annexin A8 antibody for Flow cytometry,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes Annexin A8
Tested Reactivity Hu
Tested Application FACS, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Annexin A8
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 20-61 of Human Annexin A8. (HFNPDPDAETLYKAMKGIGTNEQAIIDVLTKRSNTQRQQIAK)
Conjugation Un-conjugated
Alternate Names ANX8; VAC-beta; CH17-360D5.2; Annexin VIII; Vascular anticoagulant-beta; Annexin A8; Annexin-8

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
WB0.1 - 0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 653145 Human ANXA8

Swiss-port # P13928 Human Annexin A8

Gene Symbol ANXA8
Gene Full Name annexin A8
Background This gene encodes a member of the annexin family of evolutionarily conserved Ca2+ and phospholipid binding proteins. The encoded protein may function as an an anticoagulant that indirectly inhibits the thromboplastin-specific complex. Overexpression of this gene has been associated with acute myelocytic leukemia. A highly similar duplicated copy of this gene is found in close proximity on the long arm of chromosome 10. [provided by RefSeq, Jul 2008]
Function This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. [UniProt]
Calculated MW 37 kDa

Images (2) Click the Picture to Zoom In

  • ARG59661 anti-Annexin A8 antibody WB image

    Western blot: 50 µg of samples under reducing conditions. HeLa and A549 whole cell lysates stained with ARG59661 anti-Annexin A8 antibody at 0.5 µg/ml, overnight at 4°C.

  • ARG59661 anti-Annexin A8 antibody FACS image

    Flow Cytometry: U2OS cells were blocked with 10% normal goat serum and then stained with ARG59661 anti-Annexin A8 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.