ARG43228
anti-Annexin A5 antibody
anti-Annexin A5 antibody for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Annexin A5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Annexin A5 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to A2-D320 of Human Annexin A5. |
| Conjugation | Un-conjugated |
| Alternate Names | Placental anticoagulant protein 4; PAP-I; Thromboplastin inhibitor; Annexin V; ENX2; Lipocortin V; Anchorin CII; ANX5; Annexin-5; Annexin A5; VAC-alpha; CBP-I; PP4; HEL-S-7; Vascular anticoagulant-alpha; Endonexin II; Placental anticoagulant protein I; RPRGL3; Calphobindin I |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 36 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANXA5 |
| Gene Full Name | annexin A5 |
| Background | The Annexin 5 gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. Polymorphisms in this gene have been implicated in various obstetric complications. [provided by RefSeq, Dec 2019] |
| Function | This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. [UniProt] |
| Calculated MW | 36 kDa |
| PTM | S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. [UniProt] |
Images (12) Click the Picture to Zoom In
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ARG43228 anti-Annexin A5 antibody ICC/IF image
Immunofluorescence: RH-35 cells stained with ARG43228 anti-Annexin A5 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG43228 anti-Annexin A5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43228 anti-Annexin A5 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43228 anti-Annexin A5 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat lung, Rat brain, Rat cardiac muscle, Rat kidney, HeLa, SMMC-7721, A549 and SGC whole cell lysates stained with ARG43228 anti-Annexin A5 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG43228 anti-Annexin A5 antibody FACS image
Flow Cytometry: PC-3 cells were blocked with 10% normal goat serum and then stained with ARG43228 anti-Annexin A5 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG43228 anti-Annexin A5 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG43228 anti-Annexin A5 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG43228 anti-Annexin A5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43228 anti-Annexin A5 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43228 anti-Annexin A5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43228 anti-Annexin A5 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43228 anti-Annexin A5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43228 anti-Annexin A5 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43228 anti-Annexin A5 antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat kidney tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43228 anti-Annexin A5 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43228 anti-Annexin A5 antibody IHC-Fr image
Immunohistochemistry: Frozen section of Human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43228 anti-Annexin A5 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43228 anti-Annexin A5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43228 anti-Annexin A5 antibody at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG43228 anti-Annexin A5 antibody FACS image
Flow Cytometry: SiHa cells were blocked with 10% normal goat serum and then stained with ARG43228 anti-Annexin A5 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
