ARG63272

anti-Annexin A11 antibody

anti-Annexin A11 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes Annexin A11
Tested Reactivity Hu
Tested Application IHC-P, WB
Specificity NP_001148.1; NP_665875.1 and NP_665876.1 are varients that encode the same protein.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Annexin A11
Antigen Species Human
Immunogen YPGYPPPPGGYPP-C
Conjugation Un-conjugated
Alternate Names Calcyclin-associated annexin 50; Annexin A11; 56 kDa autoantigen; Annexin XI; Annexin-11; ANX11; CAP-50; CAP50

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
WB0.01 - 0.03 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 311 Human ANXA11

Swiss-port # P50995 Human Annexin A11

Background This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain the calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]
Research Area Signaling Transduction antibody
Calculated MW 54 kDa

Images (2) Click the Picture to Zoom In

  • ARG63272 anti-Annexin A11 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Placenta. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63272 anti-Annexin A11 antibody at 3.8 µg/ml dilution followed by AP-staining.

  • ARG63272 anti-Annexin A11 antibody WB image

    Western blot: 35 µg of HeLa (A) and Jurkat (B) cell lysates (in RIPA buffer) stained with ARG63272 anti-Annexin A11 antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.